题目：High-Throughput Characterization of Biomolecular Interactions Using a Combination of 
Molecular Microarrays and Ellipsometry-based Scanning Microscope
报告人：Prof. Xiangdong Zhu （University of California, USA）
摘要：
Microarrays of surface-immobilized macromolecules and chemical compounds are powerful tools for parallel analysis of hundreds or thousands of biomolecular binding events. For example, thousands of small molecule compounds from commercial and publicly available sources can now be printed on suitably functionalized solid surface and a protein probe of interest is allowed to react with the immobilized molecules simultaneously so that thousands of "pair-wise" biomolecular interactions can be simultaneously characterized. The microarray-based study of biomolecular interaction is particularly useful for genomic (DNA), proteomic (protein), and glycomic (carbohydrate) research as the number and variety of structures and functions of DNA, proteins, carbohydrates, and their ligands are large. Typically the detection of protein binding to small-molecule microarrays is fluorescence-based where the protein probe is labeled with a fluorescent tag either extrinsically or “intrinsically” through genetic engineering prior to the binding reaction. However the fluorescence-based detection only yields end-points of the biomolecular interactions instead of kinetics that are mechanistically relevant. Furthermore fluorescent labeling a protein probe inevitably changes the properties of the protein. It is thus sensible to develop label-free optical detection platforms with adequate sensitivity and versatility for detection of binding reactions on biomolecular microarrays. We describe an ellipsometry-based scanning microscope for high-throughput detection of molecular microarrays on functionalized glass slides for characterization of protein-molecule interactions and virus-molecule interaction on immobilized microarray. The microscope is capable of simultaneously measuring binding kinetics of 10,000 protein-molecule reactions. It has the potential to screen thousands to millions of compounds against a protein target at a rate of 100,000 compounds in one day.
Prof. Xiangdong Zhu 简介：
Professor Xiangdong Zhu received his Bachelor of Science in Physics in Peking University in China and his Ph.D. in Physics in 1989 in University of California at Berkeley in USA. From 1989 to 1993, he was an assistant Professor of Physics in University of California at Davis. He then worked as an associate Professor of Physics in University of California at Davis to 1998. Since 1998, he has been a professor of Physics in University of California at Davis. He has published nearly 100 journal articles. Moreover, he is a fellow of the American Physical Society and adjunct Professor in Institute of Physics, Chinese Academy of Sciences.
时间：2009年10月14日（星期三）上午10：00
地点：中科院物理研究所D楼210会议室
联系人：金奎娟 研究员 （Tel：82648099）
主办单位：中国科学院光物理重点实验室
